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Equipment:

The Institute for Bioanalysis has labs for chemical, microbiological and biochemical/molecular biology. The microbiological lab is approved for experiments of security level 1 (regarding BioStoffV and GenTg) and security level 2 (regarding BioStoffV). The laboratory for microbiological and biochemical/molecular biology is approved for experiments of security level 1 (regarding BioStoffV and GenTg).

All three labs have a basic equipment including biological safety cabinets, centrifuges (i.a. refrigerated), water bathes, shakers, incubators, and microscopes.

There are manifold opportunities for DNA and protein analysis, for microbial experiments as well as for chemical or physical analysis and last but not least chromatographic approaches.

Techniques:

Protein protein interaction

Phage display, biomarker development, biomarker detection, ELISA, fluorescence microscopy, identification of ligands by AP-MS and in house software PIPINO

Protein quantification
SILAC (>3000 proteins), SILAM, TMT (up to 10-plex), LFQ

Protein modification
Phosphorylation, signal peptidase cleavage sites, sulfation

Protein structure
Chemical crosslinking, H/D exchange mass spectrometry

Antibodies
Antibody development and production (GLP/GMP) (at Fraunhofer IZI department Therapy development)

Microbial communities
Characterization of phylogenetic and functional composition of microbial communities (biofilm, food stuff, biofuel, material)

Material investigation
Material resistance and colonization ability regarding microorganisms and biofilms with or without biocide

Analysis of microbial adaptation
Microbial susceptibility to antibiotics, biocides and natural vegetable/herbal substances

Analysis of human-pathogenic microorganisms
Culture collection of food associated pathogens, identification of pathogens, determination of microbial activity, qPCR of functional genes

Based stable-isotope probing (SIP)
Analysis of  complex, metabolic processes in bacteria by fatty acid-SIP, RNA-SIP or Protein-SIP, e.g. research of biocide degradation or methanogenesis Fluorescence in situ Hybridisation (FISH) Sequence based visualization of microorganisms in their habitat

Equipment

Chemical Lab

•  Orbitrap Mass Spectrometer: protein identification, protein modification, quantification, proteomic analysis

•  Nano HPLC: protein separation

•  IR spectrometer: identification of  functional groups and small molecules

•  GC-MS: identification of compounds with low molecular weight

•  Mobile GC: field measures of gaseous compounds

•  UPLC system: separation of  complex mixtures

•  ICP-MS: quantitative element analysis

Microbiological und biochemical lab

•  DNA, RNA and protein gel electrophoresis

•  Incubators

•  Autoclaves

•  High-throughput extraction methods for RNA and DNA from complex matrices

•  PCR-thermocycler: DNA  and RNA amplification

•  qPCR-Cycler for real-time PCR

•  FLUOstar Optima Microplate Reader: e.g. fluorescence-, luminescence- detection, UV/VIS-  absorbance spectra

•  Photometer

•  Different microscopes

•  Fluorescence microscope with an digital EM-CCD-camera