The Institute of Bioanalysis has labs for chemical, microbiological, biochemical/molecular biology and cell culture. The microbiological lab is approved for experiments subjected security level 1 (regarding BioStoffV and GentG) and security level 2 (regarding BioStoffV). The laboratory for microbiological and biochemical/molecular biology is approved for experiments subjected security level 1 (regarding BioStoffV and GentG).
In 2018 the Institute for Bioanalysis was extended by a lab for molecular cell biology to open the possibility for cell culture experiments.
All four labs possess basic equipment including biological safety cabinets, centrifuges (refrigerated), waterbaths, shakers, incubators, and microscopes.
There are manifold opportunities for DNA-and protein analysis, for microbial experiments as well as for chemical or physical analysis and last but not least chromatographic approaches.
Phage display, biomarker development, biomarker detection, ELISA, fluorescence microscopy, identification of ligands by AP-MS and in house software PIPINO
Protein quantification SILAC (>3000 proteins), SILAM, TMT (up to 10-plex), LFQ
Protein modification Phosphorylation, Signal peptidase cleavage sites, sulfation
Protein structure Chemical crosslinking, H/D exchange mass spectrometry
Antibodies Antibody development and production, liable to GLP/GMP (Fraunhofer IZI)
Microbial communities Characterization of phylogenetic and functional composition of microbial communities (biofilm, food stuff, biofuel, material)
Material investigation Material resistance and colonization ability regarding microorganisms and biofilms with or without biocide
Analysis of microbial adaptation Microbial susceptibility (to antibiotics, biocides and natural vegetable/herbal substances)
Analysis of human-pathogenic microorganisms Culture collection of food associated pathogens, identification of pathogens, determination of microbial activity, qPCR of functional genes
Based stable-isotope probing (SIP) Analysis of complex, metabolic processes in bacteria by fatty acid-SIP, RNA-SIP or Protein-SIP, e.g. research of biocide degradation or methanogenesis
Fluoreszenz in situ Hybridisierung (FISH) Sequence based visualisation of microorganisms in their habitat
Cell culture Primary cell culture 3D cell and organoid culture
Gene expression analysis Sequencing and microarrays siRNA and lentiviral knock down of genes for functional analysis
Fluorescence based cell staining Flow Cytometry Immunofluorescence staining, microscopy and image analysis
Drug discovery Development of cell based assay systems for drug discovery and testing Biomarker discovery Metabolomics
Epigenetics Analysis of epigenetic modification inhuman cells and tissues
Cell culture Cultivation of primary human cells and cell lines, cell based assays to monitor cellular processes, 3D cellculture techniques (e.g. human skin models), transfection of cells for manipulation (e.g. siRNA, shRNA), histology andImmunofluorescence staining and microscopy, gene expression profiling (RNA-Seq, Microarrrays, qPCR), Flow Cytometry, all standard molecular biology techniques (cloning, protein analysis etc.)
Orbitrap Mass Spectrometer: protein identification, protein modification, quantification, proteomic analysis
Nano HPLC: protein separation
IR spectrometer: identification of functional groups and small molecules
GC-MS: identification of compounds with low molecular weight
Mobile GC: field measures of gaseous compounds
UPLC system: separation of complex mixtures
ICP-MS: quantitative element analysis
Microbiological und biochemical lab
high performance flow cytometer (detecting 17 parameters)
DNA, RNA and protein gel electrophoresis
High-throughput extraction methods for RNA and DNA from complex matrices