The research unit Molecular Cell Biology focuses on tumor biology, ageing research, metabolism and development of 3D cell culture models and identification of biomarkers. Therefore multiple molecular and cell biology methods were applied, such as 3D cell culture, transcriptomics, siRNA screenings and laser scanning microscopy.
“Gesundheit messen”: Methods for evidence-based health promotion
Dehydration is a common disease and is particularly prevalent in aged patients. The project aims to develop osmotic stress model using human skin cells and identify biomarkers. In close collaboration with the Institute for Sensor and Actuator Technology (ISAT) and department of Social Work and Health we develop methods and sensors to measure biomarkers for diagnosis of acute and chronic dehydration. If you are interested in further information please visit the homepage of “ISAT-Institute of Sensor and Actuator Technology”.
Epigenetics of human skin: Analysis of H2B-Ubiquitination in human skin cells and cell culture models
Epigenetic gene regulation controls cell differentiation and play a major role during tumor development and progression. The project focus on the investigation of H2B ubiquitination during epidermal differentiation and tumor development as well as damage response of human skin cells.
Kuehne A §, Hildebrand J §, Soehle J, Wenck H, Terstegen L, Gallinat S, Knott A, Winnefeld M, Zamboni N (2016). An integrative metabolomics and transcriptomics study to identify metabolic alterations in aged skin of humans in vivo. BMC Genomics, 18(1):169, § gleichwertiger Erstautor
Knott A, Achterberg V, Smuda C, Mielke H, Sperling G, Dunckelmann K, Vogelsang A, Krüger A, Schwengler H, Behtash M, Kristof S, Diekmann H, Eisenberg T, Berroth A, Hildebrand J, Siegner R, Winnefeld M, Teuber F, Fey S, Möbius J, Retzer D, Burkhardt T, Lüttke J, Blatt T (2015). Topical treatment with coenzyme Q10-containing formulas improves skin’s Q10 level and provides antioxidative effects. Biofactors, 41(6):383-90
Kuehne A §, Emmert H §, Soehle J, Winnefeld M, Fischer F, Wenck H, Gallinat S, Terstegen L, Lucius R, Hildebrand J*, Zamboni N* (2015). Acute Activation of Oxidative Pentose Phosphate Pathway as First-Line Response to Oxidative Stress in Human Skin Cells. Molecular Cell, 59(3):359-71, § gleichwertige Erstautoren, *Corresponding Author
Hildebrand J, Grundhoff A, Gallinat S, Wenck H, Knott A (2013). MicroRNA Profiling during Human Keratinocyte Differentiation using a Quantitative Real Time PCR Method. Molecular Dermatology: Methods and Protocols, 961:193-200
Hildebrand J §, Spoerl F §, Korge S, Gallinat S, Wenck H, Deppert W, Knott A, Blatt T (2012). Cell cycle regulator Cdkn1c (p57/KIP2) shows distinct expression in epidermal differentiation. European Journal of Dermatology,22(5):694-6, § gleichwertiger Erstautor
Hildebrand J, Rütze M, Walz N, Gallinat S, Wenck H, Deppert W, Grundhoff A, Knott A (2011). A comprehensive analysis of microRNA expression during human keratinocyte differentiation in vitro and in vivo. J Invest Dermatol. 2011 Jan;131(1):20-9. doi: 10.1038/jid.2010.268
Lebrun AH §, Wunder C §, Hildebrand J §, Churin Y, Zähringer U, Lindner B, Meyer TF, Heinz E, Warnecke D. (2006). Cloning of a cholesterol-alpha-glucosyltransferase from Helicobacter pylori. J Biol Chem, Sep 22;281(38):27765-72, § gleichwertiger Erstautor